Reference & Exercise

Exercise 1: Subset your data according to the tissue type assigned.

Exercise 2: Filter the samples to remove the low count genes.

Exercise 3: Run DESeq2 package to get DE genes.

Exercise 4: Use boxplot to see the distribution of the samples. What do you observe?

Exercise 5: Visualize the results using MA-plot and PCA.

Exercise 6: Save the results to a csv file.

Exercise 7: Create a volcano plot to visualize the DE genes.

Exercise 8: Perform GO enrichment (ORA analysis) on the DE genes(UP and Down).

Exercise 9: Perform GSEA and use dot plot to visualize.

Exercise 10: Save your R script and R environment for reproducibility.

Exercise 11: Create a GitHub repository and push your code there.